POK7 Antibody: Functions, Research Applications, and Scientific Insights
Introduction
POK7 (Pokemon Family Transcription Factor 7) is a zinc-finger protein involved in gene regulation, cell differentiation, and tumorigenesis. Antibodies targeting POK7 are essential in studying its role in cancer research, neurobiology, and developmental biology.
This article explores the characteristics, research applications, and clinical significance of POK7 Antibody, incorporating references from government (.gov) and educational (.edu) sources to provide reliable scientific insights.
What is POK7 Antibody?
POK7 is a transcriptional repressor that regulates gene expression by modifying chromatin accessibility. It plays a crucial role in cellular differentiation and oncogenic pathways. POK7 antibodies are utilized in various immunological assays to detect and analyze protein expression in biological samples.
For an overview of transcription factors like POK7, visit NCBI’s Gene Database.
Product Specifications
- Host Species: Rabbit/Mouse
- Target: POK7 Protein
- Form: Monoclonal/Polyclonal
- Purity: Affinity-purified
- Buffer Composition: PBS, pH 7.4
- Preservative: 0.02% Sodium Azide
- Applications: Western Blotting, Immunohistochemistry (IHC), ELISA, Flow Cytometry
For technical specifications, refer to FDA’s antibody-based assay guidelines.
Key Research Applications
1. Cancer Research
POK7 is implicated in tumorigenesis due to its role in repressing tumor suppressor genes. Studies have linked POK7 overexpression to malignancies such as leukemia and breast cancer.
For research on POK7 in cancer, explore National Cancer Institute (NCI).
2. Neurobiology
POK7 has a potential role in neural differentiation and brain development. Researchers study POK7’s involvement in neurodegenerative diseases and neurogenesis.
For neuroscience resources, visit National Institute of Neurological Disorders and Stroke (NINDS).
3. Developmental Biology
POK7 is involved in early embryonic development, making it an essential protein for studying gene regulation in developmental biology.
Find more at National Institute of Child Health and Human Development (NICHD).
Advantages of Using POK7 Antibody
1. High Specificity and Sensitivity
POK7 antibodies provide precise detection with minimal background interference.
2. Wide Range of Applications
Compatible with Western blotting, ELISA, IHC, and flow cytometry.
3. Reliable in Cancer and Neurobiology Research
POK7’s role in multiple biological processes makes it a crucial target in various research fields.
For comparisons of commercial POK7 antibodies, visit ABRF’s Antibody Validation Resource.
Storage and Handling Recommendations
- Store at -20°C or below for long-term stability.
- Avoid repeated freeze-thaw cycles to maintain antibody integrity.
- Prepare working dilutions fresh before use.
For best practices on antibody storage, visit National Center for Biotechnology Information (NCBI).
Alternative Products and Vendors
- Cell Signaling Technology (CST): Provides validated POK7 antibodies for Western blotting.
- Abcam: Supplies a range of monoclonal and polyclonal POK7 antibodies.
- Santa Cruz Biotechnology: Offers POK7 antibodies suitable for IHC and ELISA.
For independent reviews, visit NIH’s Antibody Resource Guide.
Regulatory and Ethical Considerations
Before using antibodies in diagnostic applications, comply with regulatory standards outlined by the FDA and Office for Human Research Protections (OHRP).
Conclusion
POK7 Antibody is an invaluable tool for researchers studying gene regulation, cancer, and neurobiology. With its high specificity and versatile applications, it plays a crucial role in advancing scientific understanding of transcriptional repression.
For additional information, refer to PubMed and Centers for Disease Control and Prevention (CDC) for peer-reviewed studies on POK7-related research.