Bassoon Antibody: PerCP – A Crucial Tool in Synaptic Research
Introduction
Bassoon is a large scaffolding protein that plays an essential role in the assembly and maintenance of presynaptic active zones. It is involved in synaptic vesicle trafficking and neurotransmitter release, making it a key protein in synaptic function and neurobiology (NIH, NCBI). Researchers studying neurodegenerative diseases and synaptic plasticity often target Bassoon as a molecular marker (PubMed).
What is the Bassoon Antibody: PerCP?
The Bassoon Antibody: PerCP (Peridinin-Chlorophyll-Protein Complex Conjugate) is a fluorescently labeled antibody that allows for high-resolution imaging of synaptic structures. This antibody is widely used in flow cytometry, immunofluorescence (IF), and immunohistochemistry (IHC) applications to study synaptic organization and function (CDC, FDA).
Applications in Neuroscientific Research
- Immunohistochemistry (IHC) – Visualization of Bassoon expression in brain tissue sections (NIMH).
- Immunofluorescence (IF) – Detection of synaptic protein interactions in cultured neurons (NIH Neuroscience Blueprint).
- Flow Cytometry – Quantitative analysis of Bassoon expression in neuronal populations (NINDS).
- Western Blotting (WB) – Used to analyze Bassoon protein levels in brain lysates (NCBI Gene).
- Confocal and Super-Resolution Microscopy – High-resolution imaging of presynaptic structures (Human Protein Atlas).
Bassoon in Disease Research
- Alzheimer’s Disease: Dysfunction in Bassoon-mediated synaptic function has been linked to neurodegeneration (National Institute on Aging).
- Parkinson’s Disease: Bassoon aggregation has been identified in affected neurons (NINDS).
- Schizophrenia: Alterations in synaptic scaffolding proteins, including Bassoon, may contribute to disease pathology (NIH Mental Health Research).
Antibody Validation and Quality Control
The Bassoon Antibody: PerCP undergoes rigorous validation procedures, including knockout cell line testing, peptide blocking assays, and cross-reactivity analysis to ensure specificity (NIH Reagent Validation, FDA Antibody Validation).
Key Databases and References
- UniProtKB – Provides functional and structural data on Bassoon (UniProt).
- KEGG Pathway Database – Displays signaling pathways involving Bassoon (KEGG).
- NCBI GEO DataSets – Contains transcriptomic datasets related to Bassoon expression (GEO).
- Protein Data Bank (PDB) – 3D structural information on Bassoon (PDB).
- The Human Protein Atlas – Comprehensive immunohistochemical data on Bassoon expression (HPA).
Conclusion
The Bassoon Antibody: PerCP is an essential tool in neuroscience research, synaptic function analysis, and neurodegenerative disease studies. With its validated specificity and fluorescence-conjugated properties, it enables researchers to investigate presynaptic architecture and synaptic protein networks.
For more information on purchasing and validated protocols, refer to The Antibody Registry (Antibody Registry) or explore NIH reagent resources (NIH Reagents).