Biomol-Informatics SL Protein structure

TAF10 Rabbit Polyclonal Antibody: A Key Tool for Transcriptional Research

• Caroline
• February 28, 2025
• 0

Introduction to TAF10

TAF10 (TATA-Box Binding Protein Associated Factor 10) is a crucial component of the Transcription Factor IID (TFIID) complex, which plays a fundamental role in gene expression regulation. This protein is essential for transcription initiation by RNA polymerase II, making it a vital target for molecular biology and biomedical research.

Role of TAF10 in Transcription Regulation

TAF10 is part of the basal transcription machinery and interacts with TBP (TATA-binding protein) to facilitate the recruitment of RNA polymerase II. Studies have shown that TAF10 is indispensable for embryonic development and cellular differentiation (NCBI) (PubMed).

Furthermore, TAF10 plays a key role in histone acetylation and chromatin remodeling, which are essential for gene regulation and epigenetic modifications. Research suggests that TAF10 interacts with histone-modifying enzymes to maintain the accessibility of transcription factors to target genes (NIH Epigenomics Program).

What is the TAF10 Rabbit Polyclonal Antibody?

The TAF10 Rabbit Polyclonal Antibody is a highly specific antibody designed to detect TAF10 in various biological samples. It is widely used in Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence assays. Researchers employ this antibody to investigate transcriptional regulation, epigenetics, and disease mechanisms related to gene expression.

Applications in Research

1. Western Blotting (WB) – Used to detect TAF10 protein expression in cell lysates (NIH).
2. Immunohistochemistry (IHC) – Applied to visualize TAF10 localization in tissue sections (NCI).
3. Chromatin Immunoprecipitation (ChIP) – Helps in studying transcription factor binding sites (NCBI Gene).
4. RNA Polymerase II Complex Analysis – Essential for understanding RNA synthesis and gene expression (GenBank).
5. Co-Immunoprecipitation (Co-IP) – Used to study protein-protein interactions within the transcription machinery (National Center for Biotechnology Information).
6. Flow Cytometry – Applied in cell sorting and marker identification in TAF10-expressing cells (NIH Flow Cytometry Resource).

TAF10 and Disease Research

Dysregulation of TAF10 has been linked to various disorders, including cancer and neurodegenerative diseases. Research indicates that mutations or misexpression of TAF10 can disrupt normal transcriptional processes, leading to disease states (National Human Genome Research Institute) (CDC).

• Cancer Research: Alterations in TAF10 expression have been implicated in breast cancer and leukemia, making it a potential biomarker for diagnostics and therapeutics (NCI Cancer Genomics).
• Neurological Disorders: TAF10 dysfunction has been associated with Alzheimer’s disease, where transcriptional dysregulation contributes to neuronal degeneration (National Institute on Aging).
• Developmental Diseases: Studies show that mutations in TAF10 can affect embryonic stem cell differentiation and organ development (NIH Stem Cell Information).

Antibody Validation and Quality Control

For reliable experimental results, antibody validation is crucial. The TAF10 Rabbit Polyclonal Antibody undergoes rigorous testing using knockout models, peptide blocking assays, and cross-reactivity analysis to ensure specificity and reproducibility (FDA) (USDA).

Additionally, the antibody’s specificity is tested using mass spectrometry proteomics and orthogonal approaches, providing comprehensive quality control (NIH Antibody Validation).

Key References and Databases

• UniProtKB provides a detailed protein sequence and functional annotations of TAF10 (UniProt).
• The Human Protein Atlas offers extensive immunohistochemical data on TAF10 expression (HPA).
• Ensembl Genome Browser provides genomic insights into the TAF10 gene (Ensembl).
• KEGG Pathway Database includes TAF10-related metabolic and signaling pathways (KEGG).
• NCBI GEO DataSets contains transcriptomic and epigenomic datasets related to TAF10 research (GEO).
• Antibody Registry provides information on available antibodies for TAF10 and other targets (Antibody Registry).
• Protein Data Bank (PDB) contains structural data on TAF10 and its interacting partners (PDB).

Conclusion

The TAF10 Rabbit Polyclonal Antibody is a valuable tool for molecular biology, enabling researchers to explore transcriptional regulation, chromatin remodeling, and disease mechanisms. Its application in biomedical research, epigenetics, and cancer studies makes it an indispensable reagent for laboratories worldwide.

For more details on purchasing validated antibodies, visit repositories like The Antibody Registry (Antibody Registry) or explore protocols at NIH Reagent Resource (NIH Reagents).