SHOX2 Polyclonal Antibody: Functions, Research Applications, and Scientific Insights
Introduction
SHOX2 (Short Stature Homeobox 2) is a transcription factor crucial in skeletal development and cardiac conduction. Mutations or dysregulation of SHOX2 have been associated with congenital heart defects, short stature syndromes, and certain cancers. SHOX2 polyclonal antibodies are widely used in developmental biology, cardiology, and oncology research.
This article explores the characteristics, research applications, and clinical significance of SHOX2 Polyclonal Antibody, incorporating references from government (.gov) and educational (.edu) sources to provide reliable scientific insights.
What is SHOX2 Antibody?
SHOX2 is a key homeobox gene involved in regulating gene expression for limb and heart development. The SHOX2 polyclonal antibody is commonly used to detect and analyze SHOX2 protein expression in biological samples.
For an overview of homeobox genes like SHOX2, visit NCBI’s Gene Database.
Product Specifications
- Host Species: Rabbit/Goat
- Target: SHOX2 Protein
- Form: Polyclonal
- Purity: Affinity-purified
- Buffer Composition: PBS, pH 7.4
- Preservative: 0.02% Sodium Azide
- Applications: Western Blotting, Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP)
For technical specifications, refer to FDA’s antibody-based assay guidelines.
Key Research Applications
1. Skeletal Development Research
SHOX2 is involved in limb formation, and its mutations contribute to conditions such as Léri-Weill dyschondrosteosis and Langer mesomelic dysplasia.
Find more at National Institute of Child Health and Human Development (NICHD).
2. Cardiac Research
SHOX2 is essential for pacemaker cell development in the sinoatrial node, making it a target for studying heart arrhythmias and conduction disorders.
For research on SHOX2 in cardiology, visit National Heart, Lung, and Blood Institute (NHLBI).
3. Cancer Research
SHOX2 overexpression has been linked to lung and nasopharyngeal cancers, making it a potential biomarker for early cancer detection.
Explore research studies at National Cancer Institute (NCI).
Advantages of Using SHOX2 Antibody
1. High Specificity and Sensitivity
SHOX2 antibodies provide precise detection with minimal background interference.
2. Wide Range of Applications
Compatible with Western blotting, ELISA, IHC, and ChIP.
3. Reliable in Skeletal, Cardiac, and Cancer Research
SHOX2’s role in multiple biological processes makes it a crucial target in various research fields.
For comparisons of commercial SHOX2 antibodies, visit ABRF’s Antibody Validation Resource.
Storage and Handling Recommendations
- Store at -20°C or below for long-term stability.
- Avoid repeated freeze-thaw cycles to maintain antibody integrity.
- Prepare working dilutions fresh before use.
For best practices on antibody storage, visit National Center for Biotechnology Information (NCBI).
Alternative Products and Vendors
- Cell Signaling Technology (CST): Provides validated SHOX2 antibodies for Western blotting.
- Abcam: Supplies a range of polyclonal SHOX2 antibodies.
- Santa Cruz Biotechnology: Offers SHOX2 antibodies suitable for IHC and ELISA.
For independent reviews, visit NIH’s Antibody Resource Guide.
Regulatory and Ethical Considerations
Before using antibodies in diagnostic applications, comply with regulatory standards outlined by the FDA and Office for Human Research Protections (OHRP).
Conclusion
SHOX2 Polyclonal Antibody is an invaluable tool for researchers studying skeletal development, cardiac biology, and oncology. With its high specificity and versatile applications, it plays a crucial role in advancing scientific understanding of morphogenesis and disease mechanisms.
For additional information, refer to PubMed and Centers for Disease Control and Prevention (CDC) for peer-reviewed studies on SHOX2-related research.