Biomol-Informatics SL Protein structure

In-Depth Analysis of Anti-YFP Tag (Mouse Monoclonal) Antibody: Applications and Research Insights

• Caroline
• February 27, 2025
• 0

The Anti-YFP Tag (Mouse Monoclonal) Antibody is a crucial reagent used in molecular and cellular biology research. This antibody is highly specific for the Yellow Fluorescent Protein (YFP) tag, making it an essential tool for protein detection and localization studies (National Institutes of Health). Researchers widely use it in Western blotting, immunoprecipitation, immunofluorescence, and flow cytometry to analyze protein expression and interactions (National Library of Medicine).

Biological Significance of YFP Tag

The YFP tag is a derivative of Green Fluorescent Protein (GFP), engineered for improved brightness and photostability. It allows live-cell imaging and protein tracking in various model organisms, aiding in cellular dynamics and signaling pathway studies (National Center for Biotechnology Information). The use of YFP-tagged proteins has been instrumental in cancer biology, neurobiology, and structural biology (National Cancer Institute).

Applications in Biomedical Research

The Anti-YFP Tag (Mouse Monoclonal) Antibody is applied in multiple scientific disciplines, including:

1. Western Blotting: Enables precise detection of YFP-fusion proteins in cellular lysates (National Institute of General Medical Sciences).
2. Immunoprecipitation (IP): Facilitates protein-protein interaction studies, critical for mapping cellular pathways (National Human Genome Research Institute).
3. Fluorescence Microscopy: Aids in live-cell imaging to observe real-time protein localization (National Institute of Neurological Disorders and Stroke).
4. Flow Cytometry: Used in single-cell analysis to evaluate fluorescent-tagged proteins in large cell populations (National Institute of Allergy and Infectious Diseases).
5. CRISPR and Genetic Engineering: Assists in verifying genetically modified cell lines expressing YFP-tagged proteins (National Human Genome Research Institute).
6. Neuroscience Research: Applied in neuronal tracing and synaptic activity studies (National Institute on Aging).
7. Cancer Studies: Used to track cancer cell migration and tumor progression (National Cancer Institute).

Antibody Characteristics and Storage Conditions

The Anti-YFP Tag (Mouse Monoclonal) Antibody is validated for high sensitivity and specificity. Key features include:

• Clone: Mouse monoclonal antibody.
• Reactivity: Recognizes YFP-tagged fusion proteins.
• Purity: >95% by SDS-PAGE analysis.
• Formulation: PBS with 0.09% sodium azide (Food and Drug Administration).
• Storage: Stable at -20°C for long-term preservation (Centers for Disease Control and Prevention).
• Recommended Dilution: 1:500 to 1:5000 for Western blot and immunofluorescence applications (National Library of Medicine).

Current Research and Future Prospects

YFP-tagged proteins and their detection using Anti-YFP Tag (Mouse Monoclonal) Antibody are playing an increasing role in synthetic biology, optogenetics, and high-resolution imaging techniques (National Human Genome Research Institute). Researchers are developing advanced biosensors and optogenetic constructs that integrate YFP-tagged components for precision control in cellular systems (National Institutes of Health).

Recent advancements include:

• Super-Resolution Microscopy: Utilizing YFP-tag detection for nanometer-scale imaging (National Institute of General Medical Sciences).
• Cancer Drug Screening: Investigating YFP-tagged cancer biomarkers for real-time monitoring of drug efficacy (National Cancer Institute).
• Gene Therapy Monitoring: Using YFP-tagged reporters to assess gene expression in therapeutic applications (National Institutes of Health).

Conclusion

The Anti-YFP Tag (Mouse Monoclonal) Antibody is an indispensable tool in biomedical research, genetic engineering, and fluorescence-based imaging techniques. Its specificity and versatility make it essential for applications in protein analysis, cellular imaging, and molecular biology (National Institutes of Health). As the demand for high-precision fluorescent protein tracking continues to grow, this antibody remains a cornerstone in biotechnology and medical research (National Cancer Institute).