Hydroxyl Radical Protein Footprinting: A Mass Spectrometry-Based Structural Method for Studying the Higher Order Structure of Proteins

Hydroxyl Radical Protein Footprinting: A Mass Spectrometry-Based Structural Method for Studying the Higher Order Structure of Proteins

Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been efficiently used to analyze a plethora of protein-related questions. The tactic, which makes use of hydroxyl radicals to oxidatively modify solvent-accessible amino acids, can inform on protein interplay websites and areas of conformational change. Hydroxyl radical-based footprinting was initially developed to review nucleic acids,…

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The structure of a plant-specific partitivirus capsid reveals a unique coat protein domain architecture with an intrinsically disordered protrusion

The structure of a plant-specific partitivirus capsid reveals a unique coat protein domain architecture with an intrinsically disordered protrusion

Persistent plant viruses could also be the commonest viruses in wild vegetation. A rising physique of proof for mutualism between such viruses and their hosts, means that they play an vital position in ecology and agriculture. Right here we current the capsid construction of a plant-specific partitivirus, Pepper cryptic virus 1, at 2.9 Å decision…

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Testing machine learning techniques for general application by using protein secondary structure prediction. A brief survey with studies of pitfalls and benefits using a simple progressive learning approach

Testing machine learning techniques for general application by using protein secondary structure prediction. A brief survey with studies of pitfalls and benefits using a simple progressive learning approach

Many researchers have just lately used the prediction of protein secondary construction (native conformational states of amino acid residues) to check advances in predictive and machine studying know-how equivalent to Neural Web Deep Studying. Protein secondary construction prediction continues to be a useful instrument in analysis in biomedicine and the life sciences, however it is…

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Structure, Activity and Function of the Protein Arginine Methyltransferase 6

Structure, Activity and Function of the Protein Arginine Methyltransferase 6

Members of the protein arginine methyltransferase (PRMT) household methylate the arginine residue(s) of a number of proteins and regulate a broad spectrum of mobile features. Protein arginine methyltransferase 6 (PRMT6) is a sort I PRMT that asymmetrically dimethylates the arginine residues of quite a few substrate proteins. PRMT6 introduces uneven dimethylation modification within the histone…

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Impact of plasma reactive species on the structure and functionality of pea protein isolate

Impact of plasma reactive species on the structure and functionality of pea protein isolate

The influence of plasma-produced reactive oxygen and nitrogen species, specifically O3, NxOy, H2O2 and OH, on the construction and performance of pea protein isolate (PPI) was evaluated. Reactive species had been produced via a mixture of managed measurements and plasma therapies. Pronounced structural and useful results had been noticed upon therapy with reactive species at pH…

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