Rabbit Anti-HOXA13 Antibody: Functions, Research Applications, and Scientific Insights
Introduction
HOXA13 (Homeobox A13) is a transcription factor crucial in limb and genital development. Mutations or dysregulation of HOXA13 have been linked to congenital limb malformations and certain cancers. Rabbit anti-HOXA13 antibodies are extensively used in developmental biology, cancer research, and gene regulation studies.
This article explores the characteristics, research applications, and clinical significance of Rabbit Anti-HOXA13 Antibody, incorporating references from government (.gov) and educational (.edu) sources to provide reliable scientific insights.
What is HOXA13 Antibody?
HOXA13 is a homeobox transcription factor involved in the regulation of genes necessary for limb formation and urogenital development. The Rabbit anti-HOXA13 antibody is widely used to detect and analyze HOXA13 protein expression in biological samples.
For an overview of homeobox genes like HOXA13, visit NCBI’s Gene Database.
Product Specifications
- Host Species: Rabbit
- Target: HOXA13 Protein
- Form: Monoclonal/Polyclonal
- Purity: Affinity-purified
- Buffer Composition: PBS, pH 7.4
- Preservative: 0.02% Sodium Azide
- Applications: Western Blotting, Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP)
For technical specifications, refer to FDA’s antibody-based assay guidelines.
Key Research Applications
1. Developmental Biology
HOXA13 plays a pivotal role in limb and genital development. Mutations in HOXA13 lead to congenital disorders like hand-foot-genital syndrome.
Find more at National Institute of Child Health and Human Development (NICHD).
2. Cancer Research
HOXA13 overexpression has been observed in prostate, bladder, and colorectal cancers, making it a promising biomarker and therapeutic target.
For research on HOXA13 in cancer, explore National Cancer Institute (NCI).
3. Gene Regulation Studies
HOXA13 is a key regulator of downstream genes critical in morphogenesis. Chromatin Immunoprecipitation (ChIP) using HOXA13 antibodies helps study its binding sites and regulatory mechanisms.
For detailed gene expression data, visit ENCODE Project.
Advantages of Using HOXA13 Antibody
1. High Specificity and Sensitivity
HOXA13 antibodies provide precise detection with minimal background interference.
2. Wide Range of Applications
Compatible with Western blotting, ELISA, IHC, and ChIP.
3. Reliable in Developmental and Cancer Research
HOXA13’s role in multiple biological processes makes it a crucial target in various research fields.
For comparisons of commercial HOXA13 antibodies, visit ABRF’s Antibody Validation Resource.
Storage and Handling Recommendations
- Store at -20°C or below for long-term stability.
- Avoid repeated freeze-thaw cycles to maintain antibody integrity.
- Prepare working dilutions fresh before use.
For best practices on antibody storage, visit National Center for Biotechnology Information (NCBI).
Alternative Products and Vendors
- Cell Signaling Technology (CST): Provides validated HOXA13 antibodies for Western blotting.
- Abcam: Supplies a range of monoclonal and polyclonal HOXA13 antibodies.
- Santa Cruz Biotechnology: Offers HOXA13 antibodies suitable for IHC and ELISA.
For independent reviews, visit NIH’s Antibody Resource Guide.
Regulatory and Ethical Considerations
Before using antibodies in diagnostic applications, comply with regulatory standards outlined by the FDA and Office for Human Research Protections (OHRP).
Conclusion
Rabbit anti-HOXA13 Antibody is an invaluable tool for researchers studying developmental biology, cancer, and gene regulation. With its high specificity and versatile applications, it plays a crucial role in advancing scientific understanding of morphogenesis and oncogenesis.
For additional information, refer to PubMed and Centers for Disease Control and Prevention (CDC) for peer-reviewed studies on HOXA13-related research.