Smad2 (Phospho-Ser467) Antibody: Functions, Research Applications, and Scientific Insights
Introduction
Smad2 is a key signaling protein involved in the TGF-β (Transforming Growth Factor-Beta) pathway, which regulates cell proliferation, differentiation, and apoptosis. Phosphorylation of Smad2 at Serine 467 (Ser467) is critical for its activation and function. The Smad2 (Phospho-Ser467) Antibody is widely used in cell signaling, cancer research, and developmental biology studies.
This article explores the characteristics, research applications, and clinical significance of Smad2 (Phospho-Ser467) Antibody, incorporating references from government (.gov) and educational (.edu) sources to provide reliable scientific insights.
What is Smad2 (Phospho-Ser467) Antibody?
Smad2 is a transcriptional modulator that transduces TGF-β signals from the cell surface to the nucleus. Phosphorylation at Ser467 is a critical step for Smad2 activation and nuclear translocation, where it regulates gene expression.
For an overview of TGF-β signaling and Smad proteins, visit NCBI’s Gene Database.
Product Specifications
- Host Species: Rabbit/Goat
- Target: Smad2 (Phospho-Ser467)
- Form: Polyclonal/Monoclonal
- Purity: Affinity-purified
- Buffer Composition: PBS, pH 7.4
- Preservative: 0.02% Sodium Azide
- Applications: Western Blotting, Immunohistochemistry (IHC), ELISA, Flow Cytometry
For technical specifications, refer to FDA’s antibody-based assay guidelines.
Key Research Applications
1. Cancer Research
Dysregulation of Smad2 phosphorylation is linked to tumor progression and metastasis. Phospho-Smad2 (Ser467) antibodies help analyze tumor suppressor and oncogenic pathways.
Explore research studies at National Cancer Institute (NCI).
2. TGF-β Signaling Studies
Smad2 phosphorylation at Ser467 is essential in mediating TGF-β responses in various biological processes.
For further information, visit National Institute of General Medical Sciences (NIGMS).
3. Stem Cell and Developmental Biology
TGF-β/Smad2 signaling is crucial in embryonic development and stem cell differentiation.
Find more at National Institute of Child Health and Human Development (NICHD).
Advantages of Using Smad2 (Phospho-Ser467) Antibody
1. High Specificity and Sensitivity
Phospho-Smad2 antibodies provide precise detection with minimal background interference.
2. Wide Range of Applications
Compatible with Western blotting, ELISA, IHC, and Flow Cytometry.
3. Reliable in Cancer, TGF-β Signaling, and Developmental Research
Smad2 phosphorylation is central to multiple biological processes, making this antibody essential for targeted studies.
For comparisons of commercial Smad2 antibodies, visit ABRF’s Antibody Validation Resource.
Storage and Handling Recommendations
- Store at -20°C or below for long-term stability.
- Avoid repeated freeze-thaw cycles to maintain antibody integrity.
- Prepare working dilutions fresh before use.
For best practices on antibody storage, visit National Center for Biotechnology Information (NCBI).
Alternative Products and Vendors
- Cell Signaling Technology (CST): Provides validated Smad2 (Phospho-Ser467) antibodies for Western blotting.
- Abcam: Supplies a range of polyclonal and monoclonal Smad2 antibodies.
- Santa Cruz Biotechnology: Offers Smad2 antibodies suitable for IHC and ELISA.
For independent reviews, visit NIH’s Antibody Resource Guide.
Regulatory and Ethical Considerations
Before using antibodies in diagnostic applications, comply with regulatory standards outlined by the FDA and Office for Human Research Protections (OHRP).
Conclusion
Smad2 (Phospho-Ser467) Antibody is an invaluable tool for researchers studying TGF-β signaling, cancer progression, and developmental biology. With its high specificity and versatile applications, it plays a crucial role in advancing scientific understanding of cellular signaling mechanisms.
For additional information, refer to PubMed and Centers for Disease Control and Prevention (CDC) for peer-reviewed studies on Smad2-related research.