The structure of a plant-specific partitivirus capsid reveals a unique coat protein domain architecture with an intrinsically disordered protrusion
Persistent plant viruses could also be the commonest viruses in wild vegetation. A rising physique of proof for mutualism between such viruses and their hosts, means that they play an vital position in ecology and agriculture. Right here we current the capsid construction of a plant-specific partitivirus, Pepper cryptic virus 1, at 2.9 Å decision by Cryo-EM.
Structural options, together with the T = 1 association of 60 coat protein dimers, are shared with fungal partitiviruses and the picobirnavirus lineage of dsRNA viruses. Nonetheless, the topology of the capsid is markedly completely different with protrusions emanating from, and partly comprising, the binding interface of coat protein dimers.
We present {that a} disordered area on the apex of the protrusion just isn’t required for capsid meeting and represents a hypervariable website distinctive to, and attribute of, the plant-specific partitiviruses. These outcomes counsel a structural foundation for the acquisition of further features by partitivirus coat proteins that permits mutualistic relationships with numerous plant hosts.
Sulforaphane covalently interacts with the transglutaminase 2 most cancers upkeep protein to change its construction and suppress its exercise
Sort 2 transglutaminase (TG2) features as an vital most cancers cell survival protein in a variety of cancers together with epidermal squamous cell carcinoma. TG2 exists in open and closed conformations every of which has a definite and mutually unique exercise.
The closed conformation has GTP-binding/GTPase exercise whereas the open conformation features as a transamidase to catalyze protein-protein crosslinking. GTP-binding/GTPase exercise is required for TG2 upkeep of the aggressive most cancers phenotype.
Thus, figuring out brokers that convert TG2 from the closed to the open GTP-binding/GTPase inactive conformation is a vital most cancers prevention/remedy technique. Sulforaphane (SFN) is a vital diet-derived most cancers prevention agent that’s identified to own a reactive isothiocyanate group and has potent anticancer exercise.
Utilizing a biotin-tagged SFN analog (Biotin-ITC) and kinetic evaluation we present that SFN covalently and irreversibly binds to recombinant TG2 to inhibit transamidase exercise and shift TG2 to an open/prolonged conformation, resulting in a partial inhibition of GTP binding.
We additionally present that incubation of most cancers cells or most cancers cell extract with Biotin-ITC leads to formation of a TG2/Biotin-ITC advanced and that SFN remedy of most cancers cells inhibits TG2 transamidase exercise and shifts TG2 to an open/prolonged conformation. These findings determine TG2 as a direct SFN anticancer goal in epidermal squamous cell carcinoma.
Impact of Warmth Remedy on the Property, Construction, and Aggregation of Skim Milk Proteins
To review the mechanism of heat-induced protein aggregates, skim milk was heated at 55, 65, 75, 85, and 95°C for 30 s. Then, the sulfhydryl content material, floor hydrophobicity, and secondary construction of heat-treated skim milk have been studied. Treating skim milk at completely different temperatures induced a lower in sulfhydryl content material (75.9% at 95°C) and a rise in floor hydrophobicity (44% at 95°C) with a disrupted secondary construction containing random coil, β-sheet, and β-turn of skim milk proteins.
The change in these properties facilitated mixture formation via disulfide bonds and hydrophobicity interplay. Microstructural statement additionally confirmed the next diploma of aggregation when skim milk was heated at 85 and 95°C. The results of two-dimensional polyacrylamide gel electrophoresis demonstrated that the aggregates consisted of a excessive proportion of κ-casein, β-lactoglobulin, and different whey proteins.
Impact of ultrasonic remedy on the construction and purposeful properties of mantle proteins from scallops (Patinopecten yessoensis)
On this examine, scallop mantle protein was handled by ultrasound at completely different powers, after which analyzed by ANS fluorescent probes, round dichroism spectroscopy, endogenous fluorescence spectrum, DNTB colorimetry and in-vitro digestion mannequin to elucidate the structure-function relationship.
The outcomes indicated that ultrasound can considerably have an effect on the secondary construction of scallop mantle protein like enhancing hydrophobicity, decreasing the particle measurement, growing the relative contents of α-helix and lowering contents of β-pleated sheet, β-turn and random coil, in addition to altering intrinsic fluorescence depth with blue shift of most fluorescence peak.
However ultrasound had no impact on its main construction. Furthermore, the features of scallop mantle protein have been regulated by modifying its buildings by ultrasound. Particularly, the protein had the best efficiency in foaming property and in-vitro digestibility beneath ultrasonic energy of 100 W, oil binding capability beneath 100 W, water binding capability beneath 300 W, solubility and emulsification capability beneath 400 W, and emulsion stability beneath 600 W.
These outcomes show ultrasonic remedy has the potential to successfully enhance purposeful properties and high quality of scallop mantle protein, benefiting in complete utilization of scallop mantles.
An Integrative Method to Decide 3D Protein Constructions Utilizing Sparse Paramagnetic NMR Information and Bodily Modeling
Paramagnetic nuclear magnetic resonance (NMR) strategies have emerged as highly effective instruments for construction dedication of huge, sparsely protonated proteins. Nonetheless conventional functions face a number of challenges, together with a necessity for big datasets to offset the sparsity of restraints, the issue in accounting for the conformational heterogeneity of the spin-label, and noisy experimental knowledge.
Right here we suggest an integrative method to construction dedication combining sparse paramagnetic NMR with bodily modelling to deduce approximate protein structural ensembles. We use calmodulin in advanced with the sleek muscle myosin mild chain kinase peptide as a mannequin system.
Regardless of buying knowledge from samples labeled solely on the spine amide positions, we’re capable of produce an ensemble with a median RMSD of ∼2.eight Å from a reference X-ray crystal construction. Our method requires solely spine chemical shifts and measurements of the paramagnetic rest enhancement and residual dipolar couplings that may be obtained from sparsely labeled samples.
LZerD Protein–Protein Docking Webserver Enhanced With de novo Construction Prediction
Protein-protein docking is a useful gizmo for modeling the buildings of protein complexes which have but to be experimentally decided. Understanding the buildings of protein complexes is a key element for formulating hypotheses in biophysics relating to the purposeful mechanisms of complexes.
Protein-protein docking is a longtime method for circumstances the place the buildings of the subunits have been decided. Whereas the variety of identified buildings deposited within the Protein Information Financial institution is growing, there are nonetheless many circumstances the place the buildings of particular person proteins that customers need to dock will not be decided but.
Mouse Macrophage receptor MARCO, Marco ELISA KIT |
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| ELI-16328m | Lifescience Market | 96 Tests | EUR 865 |
Macrophage Receptor MARCO (MARCO) Antibody |
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| 20-abx135966 | Abbexa |
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Macrophage Receptor MARCO (MARCO) Antibody |
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| 20-abx322023 | Abbexa |
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Mouse MARCO shRNA Plasmid |
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| 20-abx971431 | Abbexa |
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Marco ELISA Kit| Mouse Macrophage receptor MARCO ELISA Kit |
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| EF015428 | Lifescience Market | 96 Tests | EUR 689 |
MARCO siRNA |
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| 20-abx923573 | Abbexa |
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MARCO siRNA |
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| 20-abx923574 | Abbexa |
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MARCO Antibody |
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| 1-CSB-PA880072ESR2HU | Cusabio |
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Description: A polyclonal antibody against MARCO. Recognizes MARCO from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
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MARCO antibody |
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| 70R-6455 | Fitzgerald | 50 ug | EUR 467 |
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Description: Rabbit polyclonal MARCO antibody raised against the N terminal of MARCO |
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MARCO antibody |
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| 70R-8509 | Fitzgerald | 50 ug | EUR 467 |
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Description: Affinity purified rabbit polyclonal MARCO antibody |
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Human Macrophage Receptor MARCO (MARCO) ELISA Kit |
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| 20-abx385113 | Abbexa |
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Human Macrophage receptor MARCO, MARCO ELISA KIT |
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| ELI-39124h | Lifescience Market | 96 Tests | EUR 824 |
Marco ORF Vector (Mouse) (pORF) |
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| ORF049829 | ABM | 1.0 ug DNA | EUR 506 |
MARCO Protein Vector (Mouse) (pPB-C-His) |
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| PV199314 | ABM | 500 ng | EUR 603 |
MARCO Protein Vector (Mouse) (pPB-N-His) |
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| PV199315 | ABM | 500 ng | EUR 603 |
MARCO Protein Vector (Mouse) (pPM-C-HA) |
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| PV199316 | ABM | 500 ng | EUR 603 |
MARCO Protein Vector (Mouse) (pPM-C-His) |
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| PV199317 | ABM | 500 ng | EUR 603 |
MARCO Polyclonal Antibody |
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| ES9781-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against MARCO from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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MARCO Polyclonal Antibody |
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| ES9781-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against MARCO from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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MARCO Rabbit pAb |
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| A10048-100ul | Abclonal | 100 ul | EUR 308 |
MARCO Rabbit pAb |
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| A10048-200ul | Abclonal | 200 ul | EUR 459 |
MARCO Rabbit pAb |
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| A10048-20ul | Abclonal | 20 ul | EUR 183 |
MARCO Rabbit pAb |
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| A10048-50ul | Abclonal | 50 ul | EUR 223 |
MARCO Polyclonal Antibody |
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| ABP59218-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of MARCO from Human, Mouse. This MARCO antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human MARCO protein at amino acid sequence of 380-460 |
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MARCO Polyclonal Antibody |
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| ABP59218-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of MARCO from Human, Mouse. This MARCO antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human MARCO protein at amino acid sequence of 380-460 |
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MARCO Polyclonal Antibody |
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| ABP59218-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of MARCO from Human, Mouse. This MARCO antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human MARCO protein at amino acid sequence of 380-460 |
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Anti-MARCO antibody |
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| STJ112088 | St John's Laboratory | 100 µl | EUR 277 |
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Description: The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. |
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Anti-MARCO antibody |
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| STJ190939 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Unconjugated Rabbit polyclonal to MARCO |
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MARCO Polyclonal Antibody |
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| 27273-100ul | SAB | 100ul | EUR 252 |
MARCO Polyclonal Antibody |
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| 27273-50ul | SAB | 50ul | EUR 187 |
MARCO cloning plasmid |
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| CSB-CL880072HU-10ug | Cusabio | 10ug | EUR 376 |
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Description: A cloning plasmid for the MARCO gene. |
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MARCO Blocking Peptide |
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| 33R-3464 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of MARCO antibody, catalog no. 70R-8509 |
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MARCO Blocking Peptide |
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| 33R-7477 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of MARCO antibody, catalog no. 70R-6455 |
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Mouse Anti Hamster Marco Monoclonal Antibody |
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| DMABT-47716MH | Creative Diagnostics | 0.25 mg | EUR 741 |
Marco sgRNA CRISPR Lentivector set (Mouse) |
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| K3658301 | ABM | 3 x 1.0 ug | EUR 339 |
Mouse Macrophage Receptor With Collagenous Structure (MARCO) Protein |
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| 20-abx650074 | Abbexa |
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Recombinant Macrophage Receptor With Collagenous Structure (MARCO) |
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| 4-RPC614Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Macrophage Receptor With Collagenous Structure expressed in: E.coli |
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Recombinant Macrophage Receptor With Collagenous Structure (MARCO) |
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| 4-RPC614Ra01 | Cloud-Clone |
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Description: Recombinant Rat Macrophage Receptor With Collagenous Structure expressed in: E.coli |
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Human MARCO shRNA Plasmid |
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| 20-abx955715 | Abbexa |
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MARCO Polyclonal Conjugated Antibody |
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| C27273 | SAB | 100ul | EUR 397 |
MARCO ELISA KIT|Human |
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| EF005227 | Lifescience Market | 96 Tests | EUR 689 |
Marco sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3658302 | ABM | 1.0 ug DNA | EUR 154 |
Marco sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3658303 | ABM | 1.0 ug DNA | EUR 154 |
Marco sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3658304 | ABM | 1.0 ug DNA | EUR 154 |
MARCO Protein Vector (Human) (pPB-C-His) |
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| PV025121 | ABM | 500 ng | EUR 329 |
MARCO Protein Vector (Human) (pPB-N-His) |
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| PV025122 | ABM | 500 ng | EUR 329 |
MARCO Protein Vector (Human) (pPM-C-HA) |
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| PV025123 | ABM | 500 ng | EUR 329 |
MARCO Protein Vector (Human) (pPM-C-His) |
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| PV025124 | ABM | 500 ng | EUR 329 |
MARCO Protein Vector (Rat) (pPB-C-His) |
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| PV281202 | ABM | 500 ng | EUR 603 |
MARCO Protein Vector (Rat) (pPB-N-His) |
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| PV281203 | ABM | 500 ng | EUR 603 |
MARCO Protein Vector (Rat) (pPM-C-HA) |
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| PV281204 | ABM | 500 ng | EUR 603 |
MARCO Protein Vector (Rat) (pPM-C-His) |
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| PV281205 | ABM | 500 ng | EUR 603 |
Marco ORF Vector (Rat) (pORF) |
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| ORF070301 | ABM | 1.0 ug DNA | EUR 506 |
MARCO ORF Vector (Human) (pORF) |
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| ORF006281 | ABM | 1.0 ug DNA | EUR 95 |
Rat Macrophage Receptor With Collagenous Structure (MARCO) Protein |
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| 20-abx650075 | Abbexa |
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MARCO sgRNA CRISPR Lentivector set (Human) |
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| K1270701 | ABM | 3 x 1.0 ug | EUR 339 |
Marco sgRNA CRISPR Lentivector set (Rat) |
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| K6667901 | ABM | 3 x 1.0 ug | EUR 339 |
Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse) |
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| 4-PAC614Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO) |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), Biotinylated |
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| 4-PAC614Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with Biotin. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), HRP |
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| 4-PAC614Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with HRP. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), PE |
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| 4-PAC614Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with PE. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), APC |
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| 4-PAC614Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with APC. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), Cy3 |
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| 4-PAC614Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with Cy3. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), FITC |
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| 4-PAC614Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with FITC. |
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Marco sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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| K3658305 | ABM | 3 x 1.0 ug | EUR 376 |
Macrophage Receptor With Collagenous Structure (MARCO) Antibody |
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| 20-abx131562 | Abbexa |
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Macrophage Receptor With Collagenous Structure (MARCO) Antibody |
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| 20-abx131563 | Abbexa |
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MARCO sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K1270702 | ABM | 1.0 ug DNA | EUR 154 |
MARCO sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K1270703 | ABM | 1.0 ug DNA | EUR 154 |
MARCO sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K1270704 | ABM | 1.0 ug DNA | EUR 154 |
Marco sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K6667902 | ABM | 1.0 ug DNA | EUR 154 |
Marco sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K6667903 | ABM | 1.0 ug DNA | EUR 154 |
Marco sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K6667904 | ABM | 1.0 ug DNA | EUR 154 |
MARCO 3'UTR Luciferase Stable Cell Line |
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| TU013026 | ABM | 1.0 ml | EUR 1394 |
Marco 3'UTR GFP Stable Cell Line |
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| TU262886 | ABM | 1.0 ml | Ask for price |
Marco 3'UTR GFP Stable Cell Line |
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| TU162920 | ABM | 1.0 ml | Ask for price |
Marco 3'UTR Luciferase Stable Cell Line |
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| TU212886 | ABM | 1.0 ml | Ask for price |
MARCO 3'UTR GFP Stable Cell Line |
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| TU063026 | ABM | 1.0 ml | EUR 1394 |
Marco 3'UTR Luciferase Stable Cell Line |
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| TU112920 | ABM | 1.0 ml | Ask for price |
Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAC614Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with APC-Cy7. |
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Marco sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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| K3658306 | ABM | 1.0 ug DNA | EUR 167 |
Marco sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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| K3658307 | ABM | 1.0 ug DNA | EUR 167 |
Marco sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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| K3658308 | ABM | 1.0 ug DNA | EUR 167 |
MARCO Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV622369 | ABM | 1.0 ug DNA | EUR 682 |
MARCO Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV622373 | ABM | 1.0 ug DNA | EUR 682 |
MARCO Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV622374 | ABM | 1.0 ug DNA | EUR 682 |
Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Human, Rat) |
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| 4-PAC614Ra01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Macrophage Receptor With Collagenous Structure (MARCO) |
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MARCO sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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| K1270705 | ABM | 3 x 1.0 ug | EUR 376 |
Marco sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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| K6667905 | ABM | 3 x 1.0 ug | EUR 376 |
Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Human, Rat), APC |
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| 4-PAC614Ra01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with APC. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Human, Rat), Biotinylated |
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| 4-PAC614Ra01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with Biotin. |
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Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Human, Rat), FITC |
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| 4-PAC614Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with FITC. |
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Right here, we now have built-in the AttentiveDist methodology for protein construction prediction into our LZerD webserver for protein-protein docking, which allows customers to easily submit protein sequences and procure full-complex atomic fashions, with out having to provide any construction themselves. We’ve got additional prolonged the LZerD docking interface with a symmetrical homodimer mode. The LZerD server is on the market.
