Hydroxyl Radical Protein Footprinting: A Mass Spectrometry-Based Structural Method for Studying the Higher Order Structure of Proteins
Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been efficiently used to analyze a plethora of protein-related questions. The tactic, which makes use of hydroxyl radicals to oxidatively modify solvent-accessible amino acids, can inform on protein interplay websites and areas of conformational change. Hydroxyl radical-based footprinting was initially developed to review nucleic acids,…
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The structure of a plant-specific partitivirus capsid reveals a unique coat protein domain architecture with an intrinsically disordered protrusion
Persistent plant viruses could also be the commonest viruses in wild vegetation. A rising physique of proof for mutualism between such viruses and their hosts, means that they play an vital position in ecology and agriculture. Right here we current the capsid construction of a plant-specific partitivirus, Pepper cryptic virus 1, at 2.9 Å decision…
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Testing machine learning techniques for general application by using protein secondary structure prediction. A brief survey with studies of pitfalls and benefits using a simple progressive learning approach
Many researchers have just lately used the prediction of protein secondary construction (native conformational states of amino acid residues) to check advances in predictive and machine studying know-how equivalent to Neural Web Deep Studying. Protein secondary construction prediction continues to be a useful instrument in analysis in biomedicine and the life sciences, however it is…
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Crystal structure of a MarR family protein from the psychrophilic bacterium Paenisporosarcina sp. TG-14 in complex with a lipid-like molecule
MarR household proteins regulate the transcription of a number of antibiotic-resistance genes and are broadly present in micro organism and archaea. Lately, a brand new MarR household gene was recognized by genome evaluation of the psychrophilic bacterium Paenisporosarcina sp. TG-14, which was remoted from sediment-laden basal ice in Antarctica. On this research, the crystal construction of the…
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Structure, Activity and Function of the Protein Arginine Methyltransferase 6
Members of the protein arginine methyltransferase (PRMT) household methylate the arginine residue(s) of a number of proteins and regulate a broad spectrum of mobile features. Protein arginine methyltransferase 6 (PRMT6) is a sort I PRMT that asymmetrically dimethylates the arginine residues of quite a few substrate proteins. PRMT6 introduces uneven dimethylation modification within the histone…
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Impact of plasma reactive species on the structure and functionality of pea protein isolate
The influence of plasma-produced reactive oxygen and nitrogen species, specifically O3, NxOy, H2O2 and OH, on the construction and performance of pea protein isolate (PPI) was evaluated. Reactive species had been produced via a mixture of managed measurements and plasma therapies. Pronounced structural and useful results had been noticed upon therapy with reactive species at pH…
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Fc Receptor Blocker
Overview Incubation of cells with FcR Blocking Reagent, mouse, increases the specificity of labelling with MACS® Antibodies or MACS MicroBeads and thus improves the specificity of immunofluorescent staining and the purity of magnetically isolated target cells, respectively. Detailed product information Applications The FcR Blocking Reagent, the Mouse, is used to block the unwanted binding of…
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Recombinant Human CD200 (C-6His)
Product specifications Purity >95%, by SDS-PAGE, visualized with silver staining and quantitative densitometry by Coomassie blue staining. Endotoxin level <0.10 EU per 1 μg of protein by the LAL method. Activity Measured by its binding capacity in a functional ELISA. When recombinant human CD200 R1 Fc chimera (catalog # 3414-CD) is immobilized at 2 µg…
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ELISA Kit
Description The MaxSignal HTS DON ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of deoxynivalenol. This automated solution is an end-to-end workflow platform that includes simple and comprehensive multiplexed sample preparation, ready-to-use reagents and standards, and a state-of-the-art automated high-throughput detection platform. The simplified workflow saves time, practical requirements, reagents, and lab…
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ExoQuick-TC Tissue Culture Media Exosome Precipitation Solution
Description Exosomes are 60-120 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascites fluids, tissue culture media and contain different subsets of microRNA and proteins depending on the type of cell from which they are secreted. SBI ExoQuick-TC Exosome Precipitation Reagent…
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