Biomol-Informatics SL Protein structure

CD45 / LCA (B-Cell Marker) (rPTPRC/1460), 0.2mg/mL Antibody: Essential Insights and Applications

• Caroline
• February 27, 2025
• 0

CD45, also known as the Leukocyte Common Antigen (LCA), is a transmembrane protein essential for immune cell function. It is widely used in immunohistochemistry (IHC) and flow cytometry to identify leukocytes, particularly B-cells, making it a critical marker in hematopoietic studies and clinical diagnostics (NIH).

Biological Role of CD45 / LCA

CD45 is a protein tyrosine phosphatase that regulates signaling thresholds in immune cells. Its role is vital in T-cell and B-cell receptor signaling, enabling immune system responses to pathogens and malignancies (National Cancer Institute). Specifically, it is expressed in all hematopoietic cells except mature erythrocytes and platelets (National Center for Biotechnology Information).

Clinical and Research Applications

The rPTPRC/1460 antibody, supplied at 0.2mg/mL, is crucial for identifying lymphoid malignancies, including leukemia and lymphomas. It helps distinguish between B-cell and T-cell lineage tumors, supporting diagnostic pathology (Centers for Disease Control and Prevention).

1. Leukemia and Lymphoma Diagnostics: CD45 is used in diagnosing and classifying non-Hodgkin’s lymphomas (NHL) and chronic lymphocytic leukemia (CLL) (National Institutes of Health).
2. Flow Cytometry: Essential for distinguishing hematopoietic cell subsets in both clinical and research settings (National Institute of Allergy and Infectious Diseases).
3. Immunohistochemistry (IHC): Used in pathology to differentiate between hematopoietic and non-hematopoietic tumors (National Library of Medicine).
4. Autoimmune Disease Research: CD45 is implicated in conditions such as multiple sclerosis and rheumatoid arthritis (National Multiple Sclerosis Society).
5. Translational Medicine: Investigated as a therapeutic target in immune checkpoint modulation and stem cell transplantation (U.S. Department of Health & Human Services).

Antibody Characteristics

The rPTPRC/1460 monoclonal antibody has been developed for high specificity and sensitivity. It is optimized for use in Western blot, IHC, and flow cytometry, ensuring broad applicability in research and diagnostics (Food and Drug Administration).

• Concentration: 0.2mg/mL
• Reactivity: Human, Mouse, Rat
• Clone: rPTPRC/1460
• Formulation: Supplied in PBS with 0.09% sodium azide to ensure stability (American Association of Immunologists).
• Storage Conditions: Stable at -20°C to -80°C for long-term storage (National Center for Biotechnology Information).

Research Implications and Future Perspectives

CD45-targeted studies are expanding, particularly in cancer immunotherapy and personalized medicine (National Cancer Institute). Novel approaches focus on CD45 phosphatase inhibitors for tumor immune evasion, as well as its potential role in CAR-T cell therapy (National Institute of Health).

Moreover, CD45 research is integral in understanding immune aging, where altered expression correlates with age-related immunodeficiency (National Institute on Aging).

Conclusion

CD45 / LCA (rPTPRC/1460) is an indispensable tool in hematopathology and immunology. Its broad applicability in diagnostic immunohistochemistry, leukemia/lymphoma classification, and immune signaling research makes it a valuable asset in modern biomedical science (National Institutes of Health). Researchers and clinicians can utilize this antibody to gain deeper insights into immune disorders and malignancies, paving the way for innovative therapeutic strategies (National Cancer Institute).