Recombinant Human CD200 (C-6His)

Product specifications

Purity

>95%, by SDS-PAGE, visualized with silver staining and quantitative densitometry by Coomassie blue staining.

Endotoxin level

<0.10 EU per 1 μg of protein by the LAL method.

Activity

Measured by its binding capacity in a functional ELISA. When recombinant human CD200 R1 Fc chimera (catalog # 3414-CD) is immobilized at 2 µg / ml (100 µl / well), recombinant human CD200 binds with an ED50 of 0.2-1.2 µg / ml .

Source

Chinese Hamster Ovary Cell Line, CHO Derived Human CD200 Protein
Met1-Gly232, with a C-terminal 6-His tag

Registration number: P41217

N-terminal sequence

Analysis No results were obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32.

Predicted molecular mass: 23 kDa

SDS-PAGE: 38-54 kDa, reducing conditions

What does CF mean?

CF stands for Carrier Free (CF). We generally add bovine serum albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein improves the stability of the protein, increases shelf life, and allows the recombinant protein to be stored in a more dilute concentration. The free version of the operator does not contain BSA.

Which formulation is right for me?

In general, we recommend purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, carrier-free protein is recommended for applications where the presence of BSA could interfere.

Formulation

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.

Reconstitution

Reconstitute at 500 μg / mL in PBS.

Shipping

The product is shipped at room temperature. Upon receipt, please store immediately at the recommended temperature below.

Stability and storage:

  • 12 months from the date of receipt, ≤ -20 ° C as supplied.
  • 1 month at 2 to 8 ° C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 ° C under sterile conditions after reconstitution.

Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily. The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of an Ig-like V-type domain and an Ig-like C2-type domain. A splice variant of CD200 has been described and has a truncated cytoplasmic tail.

Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely expressed, but not ubiquitous. Its receptor (CD200R) is mainly restricted to mast cells, basophils, macrophages, and dendritic cells, suggesting that myeloid cell regulation is the main function of CD200. CD200-inactivated mice are characterized by increased macrophage numbers and activation and are predisposed to autoimmune disorders. CD200 and CD200R associate through their respective N-terminal Ig-like domains.

In myeloid cells, CD200R initiates inhibitory signals after receptor-ligand contact. In T cells, however, CD200 functions as an independent costimulatory molecule of the CD28 pathway. Several additional CD200R-like molecules have been identified in humans and mice, but their ability to interact with CD200 is controversial. Several viruses encode CD200 homologues that are expressed in infected cells during the lytic phase. Like CD200 itself, viral CD200 homologues also suppress myeloid cell activity, allowing for greater viral spread.

Entrez Gene IDs

4345 (human); 17470 (mouse); 102146004 (Cynomolgus Monkey)

Alternative names

antigen identified by monoclonal MRC OX-2; CD200MOX1 antigen; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; Membrane glycoprotein OX-2; OX-2

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