Human Serum Albumin ELISA Kit

Properties

Species reactivity: human

Packaging: 96-well kit (12 strips x 8 wells)

Technique(s)

Albumin ELISA Kits: Novus Biologicals

ELISA: adequate

Input

  • serum sample type
  • plasma sample type
  • sample type cell culture supernatant(s)

Test range

  • inter-assay cv: <12%
  • intra-assay cv: <10%
  • sensitivity: 6ng/mL
  • standard curve range: 4915-1200 ng/mL

Detection method: colorimetric

Sent in: wet ice

Storage temperature: −20°C

General description

The ALB ELISA kit allows the quantitative measurement of albumin in cell culture supernatants, plasma and serum. This ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, and cell culture supernatants, urine, and/or cell lysates. and fabrics.

Application

For research use only. It should not be used in diagnostic procedures. See attached general ELISA KIT procedure (sandwich, competitive and indirect ELISA)

Biochemical/physiological actions

Human serum albumin (HSA) Elisa Kit is a non-glycosylated single chain that binds to various endogenous and exogenous ligands. It functions as a plasma carrier molecule and binds primarily to non-esterified long-chain fatty acids. Albumin also binds and transports various metabolites, such as bilirubin, steroid hormones, thyroxine, tryptophan, certain vitamins, and metal ions within the body.

It also has the ability to bind to various drugs and affects their pharmacokinetics and pharmacodynamics. HSA functions as a transporter of nitric oxide and also influences the antioxidant capacity of human serum. In cases of acute hemolysis, HSA binds to heme in the bloodstream and is transported to hemopexin, where it is reabsorbed by hepatic parenchymal cells.

Other notes

  • A sample Certificate of Analysis is available for this product.
  • Write the word sample in the text box provided for the lot number.

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