Fc Receptor Blocker
Overview
Incubation of cells with FcR Blocking Reagent, mouse, increases the specificity of labelling with MACS® Antibodies or MACS MicroBeads and thus improves the specificity of immunofluorescent staining and the purity of magnetically isolated target cells, respectively.
Detailed product information
- Applications
The FcR Blocking Reagent, the Mouse, is used to block the unwanted binding of antibodies to mouse cells that express Fc receptors, such as B cells, monocytes, and macrophages. Thus, it increases the specificity of MicroBead labelling for rare cells, eg, neural stem cells, hematopoietic stem cells, or regulatory T cells.
- Uses of Fc Block
The low-affinity receptors Fc I and Fc gIII for complexed IgG (Fc or FcR receptors) are called CD32 and CD16, respectively, and are expressed on many cell types, including B lymphocytes, NK cells, granulocytes, monocytes, macrophages, and platelets. . Some antibody preparations can bind through their Fc portions to FcR-bearing cells, resulting in high “background” staining.
Mouse BD Fc Block is a purified rat IgG 2b mouse anti-CD16 / CD32 monoclonal antibody (cat # 553141 and 553142), 1 while Rat BD Fc Block (cat # 550270 and 550271) is an anti-rat monoclonal antibody CD32. 2 Mouse and rat BD Fc Block can be used to block Fc-mediated adherence of antibodies * to mouse and rat FcRs, respectively. Furthermore, the Fc domain of anti-CD16 / anti-CD32 mAb 2.4G2 has been reported to bind to the Fc gI receptor (CD64) and block this additional FcR. 1 Since the mouse and rat BD Fc block are antigen-specific mAbs, anti-mouse CD16 / CD32 and anti-rat CD32, they can also be used as primary staining reagents for the detection of mouse and rat FcR-bearing cells, respectively.
Preincubation of mouse or rat cell suspensions with the respective BD Fc Block for several minutes, prior to staining with specific antibodies, * ensures that any observed staining is due to the interaction of the part of the antibody that binds to the antigen with an antigen on the cell surface. The following figures illustrate typical Mouse and Rat BD Fc Block applications.
Experiments using unconjugated rat anti-mouse primary antibodies or mouse anti-rat primary antibodies in the presence of BD Fc Block should be carefully designed. Second-pass reagents should be chosen that do not cross-react with BD Fc Block, rat IgG 2b, or mouse IgG 1. BD Biosciences Pharmingen offers a selection of isotype-specific anti-rat and anti-mouse immunoglobulin mAbs that can be used for this purpose.
