Crystal structure of a MarR family protein from the psychrophilic bacterium Paenisporosarcina sp. TG-14 in complex with a lipid-like molecule
MarR household proteins regulate the transcription of a number of antibiotic-resistance genes and are broadly present in micro organism and archaea. Lately, a brand new MarR household gene was recognized by genome evaluation of the psychrophilic bacterium Paenisporosarcina sp.
TG-14, which was remoted from sediment-laden basal ice in Antarctica. On this research, the crystal construction of the MarR protein from Paenisporosarcina sp. TG-14 (PaMarR) was decided at 1.6 Å decision. Within the crystal construction, a novel lipid-type compound (palmitic acid) was present in a deep cavity, which was assumed to be an effector-binding web site.
Comparative structural evaluation of homologous MarR household proteins from a mesophile and a hyperthermophile confirmed that the DNA-binding area of PaMarR exhibited comparatively excessive mobility, with a disordered area between the β1 and β2 strands.
As well as, structural comparability with different homologous advanced constructions means that this construction constitutes a conformer reworked by palmitic acid. Biochemical evaluation additionally demonstrated that PaMarR binds to cognate DNA, the place PaMarR is understood to acknowledge two putative binding websites relying on its molar focus, indicating that PaMarR binds to its cognate DNA in a stoichiometric method.
The current research offers structural data on the cold-adaptive MarR protein with an aliphatic compound as its putative effector, extending the scope of MarR household protein analysis.
AlphaFold 2: Why It Works and Its Implications for Understanding the Relationships of Protein Sequence, Construction, and Operate
AlphaFold 2 (AF2) was the star of CASP14, the final biannual construction prediction experiment. Utilizing novel deep studying, AF2 predicted the constructions of many troublesome protein targets at or close to experimental decision. Right here, we current our perspective of why AF2 works and present that it’s a very refined fold recognition algorithm that exploits the completeness of the library of single area PDB constructions.
It has additionally realized native aspect chain packing rearrangements that allow it to refine proteins to excessive decision. The advantages and limitations of its potential to foretell the constructions of many extra proteins at or near atomic element are mentioned.
Forces concerned in freeze-induced egg yolk gelation: Results of assorted bond dissociation reagents on gel properties and protein construction adjustments
Urea, sodium dodecyl sulfate (SDS) and β-mercaptoethanol (2-ME) had been used to observe the roles of hydrogen bonds, hydrophobic interactions and disulfide bonds in frozen egg yolk. Yolk samples had been ready with a denaturant, and the textural traits, turbidity properties, protein patterns and constructions had been analysed.
The outcomes confirmed that SDS or 2-ME addition to egg yolk promoted its turbidity and texture properties, however urea modified the turbidity in another way. SDS-PAGE outcomes confirmed that yolk protein patterns with urea barely diminished the quantity of excessive molecular weight substances, whereas SDS and 2-ME addition elevated the quantity.
ATR-FTIR spectroscopy revealed that the protein secondary constructions modified from ordered constructions to random coils. The feel properties had been correlated with the protein secondary construction, particularly β-sheets and β-turns. Thus, the three bond dissociation reagents induced protein denaturation. Hydrogen bonds had been the vital power affecting frozen egg yolk gelation, adopted by hydrophobic interactions and disulfide bonds.
The First Cytoplasmic Loop within the Core Construction of the ABCC1 (Multidrug Resistance Protein 1; MRP1) Transporter Accommodates A number of Amino Acids Important for Its Expression
ABCC1 (human multidrug resistance protein 1 (hMRP1)) is an ATP-binding cassette transporter which effluxes xeno- and endobiotic natural anions and confers multidrug resistance by means of energetic drug efflux. The 17 transmembrane α-helices of hMRP1 are distributed amongst three membrane spanning domains (MSD0, 1, 2) with MSD1,2 every adopted by a nucleotide binding area to type the 4-domain core construction.
Eight conserved residues within the first cytoplasmic loop (CL4) of MSD1 within the descending α-helix (Gly392, Tyr404, Arg405), the perpendicular coupling helix (Asn412, Arg415, Lys416), and the ascending α-helix (Glu422, Phe434) had been focused for mutagenesis.
Mutants with each alanine and identical cost substitutions of the coupling helix residues had been expressed in HEK cells at wild-type hMRP1 ranges and their transport exercise was solely reasonably compromised. In distinction, mutants of the flanking amino acids (G392I, Y404A, R405A/Ok, E422A/D, and F434Y) had been very poorly expressed though Y404F, E422D, and F434A had been readily expressed and transport competent.
Modeling analyses indicated that Glu422 and Arg615 may type an ion pair that may stabilize transporter expression. Nevertheless, this was not supported by change mutations E422R/R615E which did not enhance hMRP1 ranges. Further constructions accompanied by rigorous biochemical validations are wanted to higher perceive the bonding interactions essential for steady hMRP1 expression.
Construction dictates the mechanism of ligand recognition within the histidine and maltose binding proteins
Two mechanisms, induced match (IF) and conformational choice (CS), have been proposed to elucidate ligand recognition coupled conformational adjustments. The histidine binding protein (HisJ) adopts the CS mechanism, through which a pre-equilibrium is established between the open and the closed states with the ligand binding to the closed state.
Regardless of being structurally just like HisJ, the maltose binding protein (MBP) adopts the IF mechanism, through which the ligand binds the open state and induces a transition to the closed state. To know the molecular determinants of this distinction, we carried out molecular dynamics (MD) simulations of coarse-grained twin construction based mostly fashions.
We discover that intra-protein contacts distinctive to the closed state are ample to advertise the conformational transition in HisJ, indicating a CS-like mechanism. In distinction, further ligand-mimicking contacts are required to “induce” the conformational transition in MBP suggesting an IF-like mechanism.
In settlement with experiments, destabilizing modifications to 2 structural options, the backbone helix (SH) and the balancing interface (BI), current in MBP however absent in HisJ, scale back the necessity for ligand-mimicking contacts indicating that SH and BI act as structural restraints that maintain MBP within the open state.
We introduce an SH like ingredient into HisJ and observe that this will impede the conformational transition rising the significance of ligand-mimicking contacts. Equally, simultaneous mutations to BI and SH in MBP scale back the barrier to conformational transitions considerably and promote a CS-like mechanism.
) Recombinant Mouse Macrophage Receptor MARCO/MARCO (N-8His) |
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| CU63-50ug | Novoprotein | 50ug | EUR 273 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
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) MARCO Recombinant Protein (Human) |
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| RP018841 | ABM | 100 ug | Ask for price |
) MARCO Recombinant Protein (Rat) |
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| RP210899 | ABM | 100 ug | Ask for price |
 Mouse Macrophage receptor MARCO, Marco ELISA KIT |
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| ELI-16328m | Lifescience Market | 96 Tests | EUR 865 |
 ELISA Kit) Mouse Macrophage Receptor MARCO (MARCO) ELISA Kit |
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| abx389793-96tests | Abbexa | 96 tests | EUR 911 |
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 Antibody) Macrophage Receptor MARCO (MARCO) Antibody |
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| 20-abx135966 | Abbexa |
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Macrophage Receptor MARCO (MARCO) Antibody |
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| 20-abx322023 | Abbexa |
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 Mouse MARCO shRNA Plasmid |
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| 20-abx971431 | Abbexa |
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 Marco ELISA Kit| Mouse Macrophage receptor MARCO ELISA Kit |
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| EF015428 | Lifescience Market | 96 Tests | EUR 689 |
 MARCO Antibody |
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| 1-CSB-PA880072ESR2HU | Cusabio |
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Description: A polyclonal antibody against MARCO. Recognizes MARCO from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
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 MARCO antibody |
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| 70R-8509 | Fitzgerald | 50 ug | EUR 467 |
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Description: Affinity purified rabbit polyclonal MARCO antibody |
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MARCO antibody |
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| 70R-6455 | Fitzgerald | 50 ug | EUR 467 |
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Description: Rabbit polyclonal MARCO antibody raised against the N terminal of MARCO |
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 MARCO siRNA |
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| 20-abx923573 | Abbexa |
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MARCO siRNA |
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| 20-abx923574 | Abbexa |
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 ELISA Kit) Human Macrophage Receptor MARCO (MARCO) ELISA Kit |
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| 20-abx385113 | Abbexa |
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 Human Macrophage receptor MARCO, MARCO ELISA KIT |
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| ELI-39124h | Lifescience Market | 96 Tests | EUR 824 |
 (pORF)) Marco ORF Vector (Mouse) (pORF) |
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| ORF049829 | ABM | 1.0 ug DNA | EUR 506 |
 (pPB-C-His)) MARCO Protein Vector (Mouse) (pPB-C-His) |
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| PV199314 | ABM | 500 ng | EUR 603 |
 (pPB-N-His)) MARCO Protein Vector (Mouse) (pPB-N-His) |
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| PV199315 | ABM | 500 ng | EUR 603 |
 (pPM-C-HA)) MARCO Protein Vector (Mouse) (pPM-C-HA) |
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| PV199316 | ABM | 500 ng | EUR 603 |
 (pPM-C-His)) MARCO Protein Vector (Mouse) (pPM-C-His) |
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| PV199317 | ABM | 500 ng | EUR 603 |
 MARCO Polyclonal Antibody |
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| 27273-100ul | SAB | 100ul | EUR 252 |
MARCO Polyclonal Antibody |
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| 27273-50ul | SAB | 50ul | EUR 187 |
 MARCO Rabbit pAb |
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| A10048-100ul | Abclonal | 100 ul | EUR 308 |
MARCO Rabbit pAb |
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| A10048-200ul | Abclonal | 200 ul | EUR 459 |
MARCO Rabbit pAb |
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| A10048-20ul | Abclonal | 20 ul | EUR 183 |
MARCO Rabbit pAb |
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| A10048-50ul | Abclonal | 50 ul | EUR 223 |
 MARCO Blocking Peptide |
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| 33R-3464 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of MARCO antibody, catalog no. 70R-8509 |
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MARCO Blocking Peptide |
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| 33R-7477 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of MARCO antibody, catalog no. 70R-6455 |
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 MARCO cloning plasmid |
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| CSB-CL880072HU-10ug | Cusabio | 10ug | EUR 376 |
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Description: A cloning plasmid for the MARCO gene. |
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MARCO Polyclonal Antibody |
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| ABP59218-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of MARCO from Human, Mouse. This MARCO antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human MARCO protein at amino acid sequence of 380-460 |
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MARCO Polyclonal Antibody |
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| ABP59218-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of MARCO from Human, Mouse. This MARCO antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human MARCO protein at amino acid sequence of 380-460 |
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MARCO Polyclonal Antibody |
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| ABP59218-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of MARCO from Human, Mouse. This MARCO antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human MARCO protein at amino acid sequence of 380-460 |
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MARCO Polyclonal Antibody |
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| ES9781-100ul | ELK Biotech | 100ul | EUR 279 |
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Description: A Rabbit Polyclonal antibody against MARCO from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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MARCO Polyclonal Antibody |
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| ES9781-50ul | ELK Biotech | 50ul | EUR 207 |
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Description: A Rabbit Polyclonal antibody against MARCO from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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 Anti-MARCO antibody |
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| STJ112088 | St John's Laboratory | 100 µl | EUR 277 |
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Description: The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. |
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Anti-MARCO antibody |
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| STJ190939 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Unconjugated Rabbit polyclonal to MARCO |
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 Mouse Anti Hamster Marco Monoclonal Antibody |
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| DMABT-47716MH | Creative Diagnostics | 0.25 mg | EUR 741 |
) Marco sgRNA CRISPR Lentivector set (Mouse) |
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| K3658301 | ABM | 3 x 1.0 ug | EUR 339 |
 Protein) Mouse Macrophage Receptor With Collagenous Structure (MARCO) Protein |
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| 20-abx650074 | Abbexa |
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) Recombinant Macrophage Receptor With Collagenous Structure (MARCO) |
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| 4-RPC614Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Macrophage Receptor With Collagenous Structure expressed in: E.coli |
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Recombinant Macrophage Receptor With Collagenous Structure (MARCO) |
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| 4-RPC614Ra01 | Cloud-Clone |
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Description: Recombinant Rat Macrophage Receptor With Collagenous Structure expressed in: E.coli |
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 MARCO ELISA KIT|Human |
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| EF005227 | Lifescience Market | 96 Tests | EUR 689 |
 MARCO Polyclonal Conjugated Antibody |
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| C27273 | SAB | 100ul | EUR 397 |
 Human MARCO shRNA Plasmid |
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| 20-abx955715 | Abbexa |
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 (Target 1)) Marco sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3658302 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Marco sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3658303 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Marco sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3658304 | ABM | 1.0 ug DNA | EUR 154 |
 (pPB-C-His)) MARCO Protein Vector (Human) (pPB-C-His) |
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| PV025121 | ABM | 500 ng | EUR 329 |
 (pPB-N-His)) MARCO Protein Vector (Human) (pPB-N-His) |
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| PV025122 | ABM | 500 ng | EUR 329 |
 (pPM-C-HA)) MARCO Protein Vector (Human) (pPM-C-HA) |
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| PV025123 | ABM | 500 ng | EUR 329 |
 (pPM-C-His)) MARCO Protein Vector (Human) (pPM-C-His) |
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| PV025124 | ABM | 500 ng | EUR 329 |
 (pPB-C-His)) MARCO Protein Vector (Rat) (pPB-C-His) |
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| PV281202 | ABM | 500 ng | EUR 603 |
 (pPB-N-His)) MARCO Protein Vector (Rat) (pPB-N-His) |
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| PV281203 | ABM | 500 ng | EUR 603 |
 (pPM-C-HA)) MARCO Protein Vector (Rat) (pPM-C-HA) |
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| PV281204 | ABM | 500 ng | EUR 603 |
 (pPM-C-His)) MARCO Protein Vector (Rat) (pPM-C-His) |
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| PV281205 | ABM | 500 ng | EUR 603 |
 (pORF)) Marco ORF Vector (Rat) (pORF) |
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| ORF070301 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) MARCO ORF Vector (Human) (pORF) |
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| ORF006281 | ABM | 1.0 ug DNA | EUR 95 |
 Protein) Rat Macrophage Receptor With Collagenous Structure (MARCO) Protein |
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| 20-abx650075 | Abbexa |
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) Marco sgRNA CRISPR Lentivector set (Rat) |
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| K6667901 | ABM | 3 x 1.0 ug | EUR 339 |
) MARCO sgRNA CRISPR Lentivector set (Human) |
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| K1270701 | ABM | 3 x 1.0 ug | EUR 339 |
 Polyclonal Antibody (Mouse)) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse) |
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| 4-PAC614Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO) |
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) Marco sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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| K3658305 | ABM | 3 x 1.0 ug | EUR 376 |
 Polyclonal Antibody (Mouse), APC) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), APC |
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| 4-PAC614Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with APC. |
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 Polyclonal Antibody (Mouse), Biotinylated) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), Biotinylated |
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| 4-PAC614Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with Biotin. |
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 Polyclonal Antibody (Mouse), Cy3) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), Cy3 |
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| 4-PAC614Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with Cy3. |
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 Polyclonal Antibody (Mouse), FITC) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), FITC |
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| 4-PAC614Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with FITC. |
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 Polyclonal Antibody (Mouse), HRP) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), HRP |
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| 4-PAC614Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with HRP. |
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 Polyclonal Antibody (Mouse), PE) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), PE |
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| 4-PAC614Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with PE. |
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 Antibody) Macrophage Receptor With Collagenous Structure (MARCO) Antibody |
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| 20-abx131562 | Abbexa |
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Macrophage Receptor With Collagenous Structure (MARCO) Antibody |
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| 20-abx131563 | Abbexa |
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 (Target 1)) Marco sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K6667902 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Marco sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K6667903 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Marco sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K6667904 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) MARCO sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K1270702 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) MARCO sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K1270703 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) MARCO sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K1270704 | ABM | 1.0 ug DNA | EUR 154 |
 Marco 3'UTR Luciferase Stable Cell Line |
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| TU112920 | ABM | 1.0 ml | Ask for price |
 Marco 3'UTR GFP Stable Cell Line |
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| TU162920 | ABM | 1.0 ml | Ask for price |
Marco 3'UTR Luciferase Stable Cell Line |
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| TU212886 | ABM | 1.0 ml | Ask for price |
Marco 3'UTR GFP Stable Cell Line |
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| TU262886 | ABM | 1.0 ml | Ask for price |
 MARCO 3'UTR GFP Stable Cell Line |
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| TU063026 | ABM | 1.0 ml | EUR 1394 |
 MARCO 3'UTR Luciferase Stable Cell Line |
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| TU013026 | ABM | 1.0 ml | EUR 1394 |
 (Target 1)) Marco sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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| K3658306 | ABM | 1.0 ug DNA | EUR 167 |
 (Target 2)) Marco sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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| K3658307 | ABM | 1.0 ug DNA | EUR 167 |
 (Target 3)) Marco sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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| K3658308 | ABM | 1.0 ug DNA | EUR 167 |
 Polyclonal Antibody (Mouse), APC-Cy7) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAC614Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Macrophage Receptor With Collagenous Structure (MARCO). This antibody is labeled with APC-Cy7. |
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 (CMV) (pLenti-GIII-CMV)) MARCO Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV622369 | ABM | 1.0 ug DNA | EUR 682 |
 (UbC) (pLenti-GIII-UbC)) MARCO Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV622373 | ABM | 1.0 ug DNA | EUR 682 |
 (EF1a) (pLenti-GIII-EF1a)) MARCO Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV622374 | ABM | 1.0 ug DNA | EUR 682 |
) Marco sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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| K6667905 | ABM | 3 x 1.0 ug | EUR 376 |
) MARCO sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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| K1270705 | ABM | 3 x 1.0 ug | EUR 376 |
 Polyclonal Antibody (Human, Rat)) Macrophage Receptor With Collagenous Structure (MARCO) Polyclonal Antibody (Human, Rat) |
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| 4-PAC614Ra01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Macrophage Receptor With Collagenous Structure (MARCO) |
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 (CMV) (pLenti-GIII-CMV-C-term-HA)) MARCO Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV622370 | ABM | 1.0 ug DNA | EUR 682 |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) MARCO Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV622371 | ABM | 1.0 ug DNA | EUR 740 |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) MARCO Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV622372 | ABM | 1.0 ug DNA | EUR 740 |
Collectively, our outcomes present that structural restraints current within the protein construction can decide the mechanism of conformational transitions and even easy fashions that accurately seize such structural options can predict their positions. MD simulations of such fashions can thus be used, together with mutational experiments, to manage protein ligand interactions, and modulate ligand binding affinities.
