Crystal structure of a MarR family protein from the psychrophilic bacterium Paenisporosarcina sp. TG-14 in complex with a lipid-like molecule
MarR household proteins regulate the transcription of a number of antibiotic-resistance genes and are broadly present in micro organism and archaea. Lately, a brand new MarR household gene was recognized by genome evaluation of the psychrophilic bacterium Paenisporosarcina sp.
TG-14, which was remoted from sediment-laden basal ice in Antarctica. On this research, the crystal construction of the MarR protein from Paenisporosarcina sp. TG-14 (PaMarR) was decided at 1.6 Å decision. Within the crystal construction, a novel lipid-type compound (palmitic acid) was present in a deep cavity, which was assumed to be an effector-binding web site.
Comparative structural evaluation of homologous MarR household proteins from a mesophile and a hyperthermophile confirmed that the DNA-binding area of PaMarR exhibited comparatively excessive mobility, with a disordered area between the β1 and β2 strands.
As well as, structural comparability with different homologous advanced constructions means that this construction constitutes a conformer reworked by palmitic acid. Biochemical evaluation additionally demonstrated that PaMarR binds to cognate DNA, the place PaMarR is understood to acknowledge two putative binding websites relying on its molar focus, indicating that PaMarR binds to its cognate DNA in a stoichiometric method.
The current research offers structural data on the cold-adaptive MarR protein with an aliphatic compound as its putative effector, extending the scope of MarR household protein analysis.
AlphaFold 2: Why It Works and Its Implications for Understanding the Relationships of Protein Sequence, Construction, and Operate
AlphaFold 2 (AF2) was the star of CASP14, the final biannual construction prediction experiment. Utilizing novel deep studying, AF2 predicted the constructions of many troublesome protein targets at or close to experimental decision. Right here, we current our perspective of why AF2 works and present that it’s a very refined fold recognition algorithm that exploits the completeness of the library of single area PDB constructions.
It has additionally realized native aspect chain packing rearrangements that allow it to refine proteins to excessive decision. The advantages and limitations of its potential to foretell the constructions of many extra proteins at or near atomic element are mentioned.
Forces concerned in freeze-induced egg yolk gelation: Results of assorted bond dissociation reagents on gel properties and protein construction adjustments
Urea, sodium dodecyl sulfate (SDS) and β-mercaptoethanol (2-ME) had been used to observe the roles of hydrogen bonds, hydrophobic interactions and disulfide bonds in frozen egg yolk. Yolk samples had been ready with a denaturant, and the textural traits, turbidity properties, protein patterns and constructions had been analysed.
The outcomes confirmed that SDS or 2-ME addition to egg yolk promoted its turbidity and texture properties, however urea modified the turbidity in another way. SDS-PAGE outcomes confirmed that yolk protein patterns with urea barely diminished the quantity of excessive molecular weight substances, whereas SDS and 2-ME addition elevated the quantity.
ATR-FTIR spectroscopy revealed that the protein secondary constructions modified from ordered constructions to random coils. The feel properties had been correlated with the protein secondary construction, particularly β-sheets and β-turns. Thus, the three bond dissociation reagents induced protein denaturation. Hydrogen bonds had been the vital power affecting frozen egg yolk gelation, adopted by hydrophobic interactions and disulfide bonds.
The First Cytoplasmic Loop within the Core Construction of the ABCC1 (Multidrug Resistance Protein 1; MRP1) Transporter Accommodates A number of Amino Acids Important for Its Expression
ABCC1 (human multidrug resistance protein 1 (hMRP1)) is an ATP-binding cassette transporter which effluxes xeno- and endobiotic natural anions and confers multidrug resistance by means of energetic drug efflux. The 17 transmembrane α-helices of hMRP1 are distributed amongst three membrane spanning domains (MSD0, 1, 2) with MSD1,2 every adopted by a nucleotide binding area to type the 4-domain core construction.
Eight conserved residues within the first cytoplasmic loop (CL4) of MSD1 within the descending α-helix (Gly392, Tyr404, Arg405), the perpendicular coupling helix (Asn412, Arg415, Lys416), and the ascending α-helix (Glu422, Phe434) had been focused for mutagenesis.
Mutants with each alanine and identical cost substitutions of the coupling helix residues had been expressed in HEK cells at wild-type hMRP1 ranges and their transport exercise was solely reasonably compromised. In distinction, mutants of the flanking amino acids (G392I, Y404A, R405A/Ok, E422A/D, and F434Y) had been very poorly expressed though Y404F, E422D, and F434A had been readily expressed and transport competent.
Modeling analyses indicated that Glu422 and Arg615 may type an ion pair that may stabilize transporter expression. Nevertheless, this was not supported by change mutations E422R/R615E which did not enhance hMRP1 ranges. Further constructions accompanied by rigorous biochemical validations are wanted to higher perceive the bonding interactions essential for steady hMRP1 expression.
Construction dictates the mechanism of ligand recognition within the histidine and maltose binding proteins
Two mechanisms, induced match (IF) and conformational choice (CS), have been proposed to elucidate ligand recognition coupled conformational adjustments. The histidine binding protein (HisJ) adopts the CS mechanism, through which a pre-equilibrium is established between the open and the closed states with the ligand binding to the closed state.
Regardless of being structurally just like HisJ, the maltose binding protein (MBP) adopts the IF mechanism, through which the ligand binds the open state and induces a transition to the closed state. To know the molecular determinants of this distinction, we carried out molecular dynamics (MD) simulations of coarse-grained twin construction based mostly fashions.
We discover that intra-protein contacts distinctive to the closed state are ample to advertise the conformational transition in HisJ, indicating a CS-like mechanism. In distinction, further ligand-mimicking contacts are required to “induce” the conformational transition in MBP suggesting an IF-like mechanism.
In settlement with experiments, destabilizing modifications to 2 structural options, the backbone helix (SH) and the balancing interface (BI), current in MBP however absent in HisJ, scale back the necessity for ligand-mimicking contacts indicating that SH and BI act as structural restraints that maintain MBP within the open state.
We introduce an SH like ingredient into HisJ and observe that this will impede the conformational transition rising the significance of ligand-mimicking contacts. Equally, simultaneous mutations to BI and SH in MBP scale back the barrier to conformational transitions considerably and promote a CS-like mechanism.
Recombinant Macrophage Receptor With Collagenous Structure (MARCO) |
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| 4-RPC614Ra01 | Cloud-Clone |
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Description: Recombinant Rat Macrophage Receptor With Collagenous Structure expressed in: E.coli |
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MARCO Protein Vector (Mouse) (pPM-C-HA) |
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| PV199316 | ABM | 500 ng | EUR 723.6 |
MARCO Protein Vector (Mouse) (pPB-C-His) |
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| PV199314 | ABM | 500 ng | EUR 723.6 |
MARCO Protein Vector (Mouse) (pPB-N-His) |
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| PV199315 | ABM | 500 ng | EUR 723.6 |
MARCO Protein Vector (Mouse) (pPM-C-His) |
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| PV199317 | ABM | 500 ng | EUR 723.6 |
March1 Recombinant Protein (Mouse) |
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| RP149426 | ABM | 100 ug | Ask for price |
March1 Recombinant Protein (Mouse) |
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| RP149429 | ABM | 100 ug | Ask for price |
March1 Recombinant Protein (Mouse) |
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| RP149432 | ABM | 100 ug | Ask for price |
March2 Recombinant Protein (Mouse) |
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| RP149444 | ABM | 100 ug | Ask for price |
March2 Recombinant Protein (Mouse) |
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| RP149447 | ABM | 100 ug | Ask for price |
March3 Recombinant Protein (Mouse) |
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| RP149450 | ABM | 100 ug | Ask for price |
March4 Recombinant Protein (Mouse) |
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| RP149453 | ABM | 100 ug | Ask for price |
March5 Recombinant Protein (Mouse) |
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| RP149456 | ABM | 100 ug | Ask for price |
March5 Recombinant Protein (Mouse) |
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| RP149459 | ABM | 100 ug | Ask for price |
March5 Recombinant Protein (Mouse) |
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| RP149462 | ABM | 100 ug | Ask for price |
March7 Recombinant Protein (Mouse) |
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| RP149468 | ABM | 100 ug | Ask for price |
March8 Recombinant Protein (Mouse) |
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| RP149471 | ABM | 100 ug | Ask for price |
MARCH9 Recombinant Protein (Mouse) |
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| RP149474 | ABM | 100 ug | Ask for price |
MARCKS Recombinant Protein (Mouse) |
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| RP149477 | ABM | 100 ug | Ask for price |
March10 Recombinant Protein (Mouse) |
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| RP149435 | ABM | 100 ug | Ask for price |
March10 Recombinant Protein (Mouse) |
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| RP149438 | ABM | 100 ug | Ask for price |
March11 Recombinant Protein (Mouse) |
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| RP149441 | ABM | 100 ug | Ask for price |
MARCKSL1 Recombinant Protein (Mouse) |
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| RP149480 | ABM | 100 ug | Ask for price |
Mouse Macrophage Receptor MARCO (MARCO) ELISA Kit |
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| abx389793-96tests | Abbexa | 96 tests | EUR 1093.2 |
Mouse Macrophage receptor MARCO, Marco ELISA KIT |
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| ELI-16328m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse Macrophage Receptor With Collagenous Structure (MARCO) Protein |
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| 20-abx650074 | Abbexa |
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Marco ELISA Kit| Mouse Macrophage receptor MARCO ELISA Kit |
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| EF015428 | Lifescience Market | 96 Tests | EUR 826.8 |
Mouse MARCO shRNA Plasmid |
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| 20-abx971431 | Abbexa |
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MARS2 Recombinant Protein (Mouse) |
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| RP149516 | ABM | 100 ug | Ask for price |
MARVELD1 Recombinant Protein (Mouse) |
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| RP149519 | ABM | 100 ug | Ask for price |
MARVELD2 Recombinant Protein (Mouse) |
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| RP149522 | ABM | 100 ug | Ask for price |
MARVELD2 Recombinant Protein (Mouse) |
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| RP149525 | ABM | 100 ug | Ask for price |
MARVELD3 Recombinant Protein (Mouse) |
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| RP149528 | ABM | 100 ug | Ask for price |
MARVELD3 Recombinant Protein (Mouse) |
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| RP149531 | ABM | 100 ug | Ask for price |
MARC1 Recombinant Protein (Rat) |
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| RP210866 | ABM | 100 ug | Ask for price |
MARC2 Recombinant Protein (Rat) |
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| RP210869 | ABM | 100 ug | Ask for price |
MARCO Protein Vector (Rat) (pPM-C-HA) |
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| PV281204 | ABM | 500 ng | EUR 723.6 |
MARC1 Recombinant Protein (Human) |
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| RP069942 | ABM | 100 ug | Ask for price |
MARC2 Recombinant Protein (Human) |
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| RP069945 | ABM | 100 ug | Ask for price |
MARCO Protein Vector (Rat) (pPB-C-His) |
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| PV281202 | ABM | 500 ng | EUR 723.6 |
MARCO Protein Vector (Rat) (pPB-N-His) |
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| PV281203 | ABM | 500 ng | EUR 723.6 |
MARCO Protein Vector (Rat) (pPM-C-His) |
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| PV281205 | ABM | 500 ng | EUR 723.6 |
MARCO Protein Vector (Human) (pPM-C-HA) |
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| PV025123 | ABM | 500 ng | EUR 394.8 |
MARCO Protein Vector (Human) (pPB-C-His) |
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| PV025121 | ABM | 500 ng | EUR 394.8 |
MARCO Protein Vector (Human) (pPB-N-His) |
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| PV025122 | ABM | 500 ng | EUR 394.8 |
MARCO Protein Vector (Human) (pPM-C-His) |
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| PV025124 | ABM | 500 ng | EUR 394.8 |
Macrophage Receptor MARCO (MARCO) Antibody |
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| 20-abx322023 | Abbexa |
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Macrophage Receptor MARCO (MARCO) Antibody |
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| 20-abx135966 | Abbexa |
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Marco ORF Vector (Mouse) (pORF) |
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| ORF049829 | ABM | 1.0 ug DNA | EUR 607.2 |
March1 Recombinant Protein (Rat) |
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| RP210872 | ABM | 100 ug | Ask for price |
March2 Recombinant Protein (Rat) |
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| RP210878 | ABM | 100 ug | Ask for price |
March3 Recombinant Protein (Rat) |
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| RP210881 | ABM | 100 ug | Ask for price |
March5 Recombinant Protein (Rat) |
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| RP210884 | ABM | 100 ug | Ask for price |
March7 Recombinant Protein (Rat) |
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| RP210887 | ABM | 100 ug | Ask for price |
March8 Recombinant Protein (Rat) |
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| RP210890 | ABM | 100 ug | Ask for price |
MARCH9 Recombinant Protein (Rat) |
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| RP210893 | ABM | 100 ug | Ask for price |
March11 Recombinant Protein (Rat) |
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| RP210875 | ABM | 100 ug | Ask for price |
MARCKSL1 Recombinant Protein (Rat) |
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| RP210896 | ABM | 100 ug | Ask for price |
March5 Recombinant Protein (Human) |
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| RP018829 | ABM | 100 ug | Ask for price |
March8 Recombinant Protein (Human) |
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| RP018832 | ABM | 100 ug | Ask for price |
March2 Recombinant Protein (Human) |
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| RP041239 | ABM | 100 ug | Ask for price |
MARCKS Recombinant Protein (Human) |
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| RP041242 | ABM | 100 ug | Ask for price |
March1 Recombinant Protein (Human) |
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| RP069948 | ABM | 100 ug | Ask for price |
March3 Recombinant Protein (Human) |
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| RP069954 | ABM | 100 ug | Ask for price |
March4 Recombinant Protein (Human) |
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| RP069957 | ABM | 100 ug | Ask for price |
March7 Recombinant Protein (Human) |
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| RP069963 | ABM | 100 ug | Ask for price |
MARCH9 Recombinant Protein (Human) |
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| RP069966 | ABM | 100 ug | Ask for price |
TAGAP Recombinant Protein (Mouse) (Recombinant- Tag) |
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| RP177155 | ABM | 100 ug | Ask for price |
TAGLN Recombinant Protein (Mouse) (Recombinant- Tag) |
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| RP177161 | ABM | 100 ug | Ask for price |
Recombinant Mouse EGF Protein |
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| RP00628 | Abclonal | 10 μg | EUR 147.6 |
Recombinant Mouse SCF Protein |
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| RP01055 | Abclonal | 10 μg | EUR 242.4 |
Recombinant Mouse LIF Protein |
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| R01400-1 | BosterBio | 100ug/vial | EUR 352.8 |
Recombinant MARCKS Related Protein (MARCKSL1) |
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| 4-RPL709Hu01 | Cloud-Clone |
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Description: Recombinant Human MARCKS Related Protein expressed in: E.coli |
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CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag) |
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| RP126449 | ABM | 100 ug | Ask for price |
CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag) |
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| RP126452 | ABM | 100 ug | Ask for price |
CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag) |
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| RP126455 | ABM | 100 ug | Ask for price |
Tagap1 Recombinant Protein (Mouse) (Recombinant Tag) |
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| RP177158 | ABM | 100 ug | Ask for price |
TAGLN2 Recombinant Protein (Mouse) (Recombinant Tag) |
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| RP177164 | ABM | 100 ug | Ask for price |
TAGLN3 Recombinant Protein (Mouse) (Recombinant Tag) |
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| RP177167 | ABM | 100 ug | Ask for price |
Recombinant Mouse C1s1 Protein |
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| CTP-031 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1s1 protein with a N-terminal tag or C-terminal tag expressed in E. coli, yeast, insect, or mammalian cells. |
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Recombinant Mouse C1s2 Protein |
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| CTP-032 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1s2 protein with a N-terminal tag or C-terminal tag expressed in E. coli, yeast, insect, or mammalian cells. |
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Recombinant Mouse C4bp Protein |
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| CTP-063 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C4bp protein with a N-terminal tag or C-terminal tag expressed in E. Coli, yeast, insect, or mammalian cell. |
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Recombinant Mouse FGF2 Protein |
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| R00121-1 | BosterBio | 100ug/vial | EUR 352.8 |
Recombinant Mouse CD28 Protein |
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| RP01211 | Abclonal | 10 μg | EUR 188.4 |
Recombinant Mouse FGF7 Protein |
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| R01931-1 | BosterBio | 100ug/vial | EUR 352.8 |
March10 Recombinant Protein (Human) |
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| RP018826 | ABM | 100 ug | Ask for price |
March11 Recombinant Protein (Human) |
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| RP069951 | ABM | 100 ug | Ask for price |
Recombinant Mouse C1ql1 Protein |
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| CTP-018 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1ql1 protein with a N-terminal tag or C-terminal tag expressed inE. Coli, yeast, insect, or mammalian cell. |
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Recombinant Mouse EphB2 Protein |
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| RP01156 | Abclonal | 10 μg | EUR 186 |
Recombinant Mouse EphB2 Protein |
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| RP01191 | Abclonal | 10 μg | EUR 178.8 |
Recombinant Mouse PCSK9 Protein |
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| RP01200 | Abclonal | 10 μg | EUR 242.4 |
MARCKSL1 Recombinant Protein (Human) |
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| RP018835 | ABM | 100 ug | Ask for price |
MARCKSL1 Recombinant Protein (Human) |
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| RP018838 | ABM | 100 ug | Ask for price |
MARCKSP1 Recombinant Protein (Human) |
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| RP069969 | ABM | 100 ug | Ask for price |
Recombinant Mouse CXCL16 Protein |
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| RP01061 | Abclonal | 10 μg | EUR 242.4 |
Rat Macrophage Receptor With Collagenous Structure (MARCO) Protein |
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| 20-abx650075 | Abbexa |
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Recombinant Mouse C1qtnf1 Protein |
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| CTP-153 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1qtnf1 protein with a N-terminal tag or C-terminal tag expressed in E. Coli, yeast, insect, or mammalian cell. |
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Recombinant Mouse C1QTNF4 Protein |
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| CTP-167 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1QTNF4 protein with a N-terminal tag or C-terminal tag expressed in E. Coli, yeast, insect, or mammalian cell. |
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Recombinant Mouse C1qtnf7 Protein |
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| CTP-180 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1qtnf7 protein with a N-terminal tag or C-terminal tag expressed in E. Coli, yeast, insect, or mammalian cell. |
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Recombinant Mouse C1qtnf9 Protein |
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| CTP-188 | Creative Biolabs | 100ug | Ask for price |
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Description: A recombinant mouse C1qtnf9 protein with a N-terminal tag or C-terminal tag expressed in E. Coli, yeast, insect, or mammalian cell. |
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100043387 Recombinant Protein (Mouse) |
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| RP109667 | ABM | 100 ug | Ask for price |
XK Recombinant Protein (Mouse) |
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| RP185816 | ABM | 100 ug | Ask for price |
CS Recombinant Protein (Mouse) |
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| RP126215 | ABM | 100 ug | Ask for price |
F2 Recombinant Protein (Mouse) |
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| RP132623 | ABM | 100 ug | Ask for price |
F3 Recombinant Protein (Mouse) |
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| RP132638 | ABM | 100 ug | Ask for price |
F7 Recombinant Protein (Mouse) |
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| RP132650 | ABM | 100 ug | Ask for price |
F9 Recombinant Protein (Mouse) |
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| RP132671 | ABM | 100 ug | Ask for price |
T Recombinant Protein (Mouse) |
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| RP176954 | ABM | 100 ug | Ask for price |
T2 Recombinant Protein (Mouse) |
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| RP176957 | ABM | 100 ug | Ask for price |
TH Recombinant Protein (Mouse) |
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| RP178520 | ABM | 100 ug | Ask for price |
GC Recombinant Protein (Mouse) |
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| RP136085 | ABM | 100 ug | Ask for price |
Gh Recombinant Protein (Mouse) |
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| RP136409 | ABM | 100 ug | Ask for price |
HP Recombinant Protein (Mouse) |
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| RP142268 | ABM | 100 ug | Ask for price |
IK Recombinant Protein (Mouse) |
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| RP143318 | ABM | 100 ug | Ask for price |
KY Recombinant Protein (Mouse) |
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| RP146723 | ABM | 100 ug | Ask for price |
MB Recombinant Protein (Mouse) |
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| RP149624 | ABM | 100 ug | Ask for price |
MB Recombinant Protein (Mouse) |
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| RP149627 | ABM | 100 ug | Ask for price |
MB Recombinant Protein (Mouse) |
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| RP149630 | ABM | 100 ug | Ask for price |
Qk Recombinant Protein (Mouse) |
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| RP166076 | ABM | 100 ug | Ask for price |
Qk Recombinant Protein (Mouse) |
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| RP166079 | ABM | 100 ug | Ask for price |
Qk Recombinant Protein (Mouse) |
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| RP166082 | ABM | 100 ug | Ask for price |
C2 Recombinant Protein (Mouse) |
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| RP120329 | ABM | 100 ug | Ask for price |
C9 Recombinant Protein (Mouse) |
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| RP120488 | ABM | 100 ug | Ask for price |
a Recombinant Protein (Mouse) |
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| RP112919 | ABM | 100 ug | Ask for price |
CTAG2 Recombinant Protein (Mouse) (Recombinant-P Tag) |
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| RP126446 | ABM | 100 ug | Ask for price |
Bax Mouse Recombinant Protein |
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| PROTQ07813 | BosterBio | Regular: 10ug | EUR 380.4 |
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Description: Bax Mouse Recombinant amino acid 38-171 produced in E.Coli is a single, non-glycosylated polypeptide chain. The Mouse Bax is purified by proprietary chromatographic techniques. |
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VTN Recombinant Protein (Mouse) |
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| RP185045 | ABM | 100 ug | Ask for price |
WAC Recombinant Protein (Mouse) |
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| RP185087 | ABM | 100 ug | Ask for price |
WAC Recombinant Protein (Mouse) |
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| RP185090 | ABM | 100 ug | Ask for price |
Wap Recombinant Protein (Mouse) |
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| RP185093 | ABM | 100 ug | Ask for price |
WAS Recombinant Protein (Mouse) |
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| RP185111 | ABM | 100 ug | Ask for price |
WLS Recombinant Protein (Mouse) |
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| RP185627 | ABM | 100 ug | Ask for price |
WRB Recombinant Protein (Mouse) |
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| RP185720 | ABM | 100 ug | Ask for price |
WT1 Recombinant Protein (Mouse) |
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| RP185747 | ABM | 100 ug | Ask for price |
Xlr Recombinant Protein (Mouse) |
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| RP185843 | ABM | 100 ug | Ask for price |
XPA Recombinant Protein (Mouse) |
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| RP185873 | ABM | 100 ug | Ask for price |
YY1 Recombinant Protein (Mouse) |
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| RP186125 | ABM | 100 ug | Ask for price |
YY2 Recombinant Protein (Mouse) |
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| RP186128 | ABM | 100 ug | Ask for price |
ZFX Recombinant Protein (Mouse) |
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| RP187991 | ABM | 100 ug | Ask for price |
ZFX Recombinant Protein (Mouse) |
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| RP187994 | ABM | 100 ug | Ask for price |
Collectively, our outcomes present that structural restraints current within the protein construction can decide the mechanism of conformational transitions and even easy fashions that accurately seize such structural options can predict their positions. MD simulations of such fashions can thus be used, together with mutational experiments, to manage protein ligand interactions, and modulate ligand binding affinities.
